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human integrin αvβ5  (R&D Systems)


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    R&D Systems human integrin αvβ5
    Human Integrin αvβ5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+integrin+%CE%B1v%CE%B25+protein/us12326457-298-27-30?v=R%26D+Systems
    Average 93 stars, based on 8 article reviews
    human integrin αvβ5 - by Bioz Stars, 2026-07
    93/100 stars

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    A. Representative curve of cell index measurement for rat VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue). B . Quantification of the rate of VSMC adhesion on gelatin coating without or with ANGPTL6 (1-4 ng/mL) using the slope of the cell index curve in its linear part (One-way ANOVA; *** P < 0.001). C. Representative images of the evan blue staining of rat VSMC that transmigrated across a porous membrane coated with gelatin containing or not ANGPTL6 after 24 h (left) and the corresponding quantification of the area covered by rat VSMC (normalized to the gelatin alone condition, Wilcoxon t -test) D. Representative curve of cell index measurement for human VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue) (left) and corresponding quantification of the rate of adhesion using the slope of the cell index curve in its linear part (Wilcoxon t -test; * P <0.05). F. Single-cycle kinetic SPR analysis of the ANGPTL6 interaction with <t>αVβ5</t> or α5β1 integrins (12.5 nM; 25 nM; 50 nM; 100nM and 200 nM) or fibronectin (62.5 nM; 125 nM; 250 nM; 500 nM and 1000 nM). The red line represents the kinetic global fit of the ANGPTL6-αVβ5 interaction using 1:1 Langmuir model.
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    A. Representative curve of cell index measurement for rat VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue). B . Quantification of the rate of VSMC adhesion on gelatin coating without or with ANGPTL6 (1-4 ng/mL) using the slope of the cell index curve in its linear part (One-way ANOVA; *** P < 0.001). C. Representative images of the evan blue staining of rat VSMC that transmigrated across a porous membrane coated with gelatin containing or not ANGPTL6 after 24 h (left) and the corresponding quantification of the area covered by rat VSMC (normalized to the gelatin alone condition, Wilcoxon t -test) D. Representative curve of cell index measurement for human VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue) (left) and corresponding quantification of the rate of adhesion using the slope of the cell index curve in its linear part (Wilcoxon t -test; * P <0.05). F. Single-cycle kinetic SPR analysis of the ANGPTL6 interaction with <t>αVβ5</t> or α5β1 integrins (12.5 nM; 25 nM; 50 nM; 100nM and 200 nM) or fibronectin (62.5 nM; 125 nM; 250 nM; 500 nM and 1000 nM). The red line represents the kinetic global fit of the ANGPTL6-αVβ5 interaction using 1:1 Langmuir model.
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    A. Representative curve of cell index measurement for rat VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue). B . Quantification of the rate of VSMC adhesion on gelatin coating without or with ANGPTL6 (1-4 ng/mL) using the slope of the cell index curve in its linear part (One-way ANOVA; *** P < 0.001). C. Representative images of the evan blue staining of rat VSMC that transmigrated across a porous membrane coated with gelatin containing or not ANGPTL6 after 24 h (left) and the corresponding quantification of the area covered by rat VSMC (normalized to the gelatin alone condition, Wilcoxon t -test) D. Representative curve of cell index measurement for human VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue) (left) and corresponding quantification of the rate of adhesion using the slope of the cell index curve in its linear part (Wilcoxon t -test; * P <0.05). F. Single-cycle kinetic SPR analysis of the ANGPTL6 interaction with <t>αVβ5</t> or α5β1 integrins (12.5 nM; 25 nM; 50 nM; 100nM and 200 nM) or fibronectin (62.5 nM; 125 nM; 250 nM; 500 nM and 1000 nM). The red line represents the kinetic global fit of the ANGPTL6-αVβ5 interaction using 1:1 Langmuir model.
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    A. Representative curve of cell index measurement for rat VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue). B . Quantification of the rate of VSMC adhesion on gelatin coating without or with ANGPTL6 (1-4 ng/mL) using the slope of the cell index curve in its linear part (One-way ANOVA; *** P < 0.001). C. Representative images of the evan blue staining of rat VSMC that transmigrated across a porous membrane coated with gelatin containing or not ANGPTL6 after 24 h (left) and the corresponding quantification of the area covered by rat VSMC (normalized to the gelatin alone condition, Wilcoxon t -test) D. Representative curve of cell index measurement for human VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue) (left) and corresponding quantification of the rate of adhesion using the slope of the cell index curve in its linear part (Wilcoxon t -test; * P <0.05). F. Single-cycle kinetic SPR analysis of the ANGPTL6 interaction with <t>αVβ5</t> or α5β1 integrins (12.5 nM; 25 nM; 50 nM; 100nM and 200 nM) or fibronectin (62.5 nM; 125 nM; 250 nM; 500 nM and 1000 nM). The red line represents the kinetic global fit of the ANGPTL6-αVβ5 interaction using 1:1 Langmuir model.
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    A. Representative curve of cell index measurement for rat VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue). B . Quantification of the rate of VSMC adhesion on gelatin coating without or with ANGPTL6 (1-4 ng/mL) using the slope of the cell index curve in its linear part (One-way ANOVA; *** P < 0.001). C. Representative images of the evan blue staining of rat VSMC that transmigrated across a porous membrane coated with gelatin containing or not ANGPTL6 after 24 h (left) and the corresponding quantification of the area covered by rat VSMC (normalized to the gelatin alone condition, Wilcoxon t -test) D. Representative curve of cell index measurement for human VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue) (left) and corresponding quantification of the rate of adhesion using the slope of the cell index curve in its linear part (Wilcoxon t -test; * P <0.05). F. Single-cycle kinetic SPR analysis of the ANGPTL6 interaction with <t>αVβ5</t> or α5β1 integrins (12.5 nM; 25 nM; 50 nM; 100nM and 200 nM) or fibronectin (62.5 nM; 125 nM; 250 nM; 500 nM and 1000 nM). The red line represents the kinetic global fit of the ANGPTL6-αVβ5 interaction using 1:1 Langmuir model.
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    A. Representative curve of cell index measurement for rat VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue). B . Quantification of the rate of VSMC adhesion on gelatin coating without or with ANGPTL6 (1-4 ng/mL) using the slope of the cell index curve in its linear part (One-way ANOVA; *** P < 0.001). C. Representative images of the evan blue staining of rat VSMC that transmigrated across a porous membrane coated with gelatin containing or not ANGPTL6 after 24 h (left) and the corresponding quantification of the area covered by rat VSMC (normalized to the gelatin alone condition, Wilcoxon t -test) D. Representative curve of cell index measurement for human VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue) (left) and corresponding quantification of the rate of adhesion using the slope of the cell index curve in its linear part (Wilcoxon t -test; * P <0.05). F. Single-cycle kinetic SPR analysis of the ANGPTL6 interaction with αVβ5 or α5β1 integrins (12.5 nM; 25 nM; 50 nM; 100nM and 200 nM) or fibronectin (62.5 nM; 125 nM; 250 nM; 500 nM and 1000 nM). The red line represents the kinetic global fit of the ANGPTL6-αVβ5 interaction using 1:1 Langmuir model.

    Journal: bioRxiv

    Article Title: ANGPTL6 variant induces cerebral vascular dysfunction and predisposes to intracranial aneurysm in mice

    doi: 10.1101/2025.01.10.632391

    Figure Lengend Snippet: A. Representative curve of cell index measurement for rat VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue). B . Quantification of the rate of VSMC adhesion on gelatin coating without or with ANGPTL6 (1-4 ng/mL) using the slope of the cell index curve in its linear part (One-way ANOVA; *** P < 0.001). C. Representative images of the evan blue staining of rat VSMC that transmigrated across a porous membrane coated with gelatin containing or not ANGPTL6 after 24 h (left) and the corresponding quantification of the area covered by rat VSMC (normalized to the gelatin alone condition, Wilcoxon t -test) D. Representative curve of cell index measurement for human VSMC adhering on gelatin coating without (grey) and with ANGPTL6 (4 ng/mL, blue) (left) and corresponding quantification of the rate of adhesion using the slope of the cell index curve in its linear part (Wilcoxon t -test; * P <0.05). F. Single-cycle kinetic SPR analysis of the ANGPTL6 interaction with αVβ5 or α5β1 integrins (12.5 nM; 25 nM; 50 nM; 100nM and 200 nM) or fibronectin (62.5 nM; 125 nM; 250 nM; 500 nM and 1000 nM). The red line represents the kinetic global fit of the ANGPTL6-αVβ5 interaction using 1:1 Langmuir model.

    Article Snippet: SPR was used to assess the interaction between fibronectin (ECM001, Sigma-aldrich), αVβ5 integrin (2528-AV, R&D systems) and α5β1 integrin (3230-A5, R&D systems) with ANGPTL6 (CSB-MP822824HU, Cusabio) immobilized onto CM5 (1000 RU; GE Healthcare) using a Biacore T200 (GE Healthcare).

    Techniques: Staining, Membrane